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KMID : 0370219960400020193
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Yakhak Hoeji
1996 Volume.40 No. 2 p.193 ~ p.201
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The Spectroscopic Study on the Role of C-terminal Region of T4 endonuclease V in the Interaction with DNA; NMR and Fluorescence experiment
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유준석/Yu JS
임형미/임후강/신정휴/이봉진/Lihm HM/Ihm HK/Shin JH/Lee BJ
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Abstract
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In order to study the role of C-terminal aromatic region of T4 endonuclease V in the interaction with substrate DNA, NMR and Fluorescence spectrum were recorded. Analysis of fluorescence emission spectra showed that C-terminal region of T4 endonuclease V is in or very near the binding site. In the HSQC spectrum of 15N-Tyr-labeled T4 endonuclease V*DNA complex, the broadening of a peak was observed. It is presumed that this peak corresponds to one among three tyrosine residues which belong to the WYKYY segment of C-terminal region of T4 endonuclease V. Interactions of peptide fragments consisting of C-terminal residues of T4 endonuclease V with DNAs(TT-, T^T-DNA) were investigated by NMR and Fluorescence experiment. The results suggest that two peptide fragments themselves bind to DNAs and their binding pattern is not an intercalation mode.
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KEYWORD
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